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licB [2018-07-26 14:38:24]
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licB [2018-07-26 14:38:24]

trigger enzyme
locus
BSU38590
pI
6.31
mw
10.82 kDa
protein length
102 aa Sequence Blast
gene length
306 bp Sequence Blast
function
lichenan uptake and phosphorylation, control of LicR activity
product
trigger enzyme
essential
no
ec
2.7.1.69
synonyms
celA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.4|Control of PRD-type regulators]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.1|Trigger enzymes of the PTS that control the activity of PRD-containing transcription factors]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • Gene

    Coordinates
    3,961,566 → 3,961,874

    The protein

    Catalyzed reaction/ biological activity

  • Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)

    • Protein family

  • lactose permease (Lac) EIIB family [Pubmed|10627040]

    • Paralogous protein(s)

  • [protein|00A4A90685CED4AD46829C45718CF26F1D62F5D3|GmuB]

    • Modification

  • phosphorylation on Ser-37 [Pubmed|17218307]

    • Structure

  • [PDB|1e2b] (from ''E. coli'', 40% identity) [Pubmed|9041631]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|65FC7F4ED92F747DB7BC5672DCA2D2320A471134|licB]-[gene|83A7C160B0301A2B51B65478D99ACB753D67AF74|licC]-[gene|E8B6C8E722BEE866878CDD816B7828C132F94FCD|licA]-[gene|84D8AA0AFBF46273F6B4198B49440D55470FEC0F|licH]
    description
    [Pubmed|8990303]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8990303], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|8990303], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|E087ABA705CE94E2CAD44A0F2FB265B0F7B04399|LicR]: activation, [Pubmed|8990303], in [regulon|E087ABA705CE94E2CAD44A0F2FB265B0F7B04399|LicR regulon]

    • regulation

  • carbon catabolite repression ([protein|search|CcpA]) [Pubmed|8990303]
    • view in new tab

    Biological materials

    Mutant

  • BKE38590 (Δ[gene|65FC7F4ED92F747DB7BC5672DCA2D2320A471134|licB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAAGACCCCCTAGA, downstream forward: _UP4_GTGTCATAGAGAGGGGCGGG
  • BKK38590 (Δ[gene|65FC7F4ED92F747DB7BC5672DCA2D2320A471134|licB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAAGACCCCCTAGA, downstream forward: _UP4_GTGTCATAGAGAGGGGCGGG

    • References

  • 19087206,8990303,16872404,10438772,10627040,17218307,8990303,9041631